ANA: Cell cycle related (Mitotic) Patterns
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Centriole |
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| Nucleus | Mitotic | Description | |
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Resting cells:
Mitotic region: Disease (antigens): |
One dot near the edge of the nucleus is visiable
Staining of the poles of the mitotic spindle apparatus appear as two dots Raynaud’s, scleroderma, Sjögren’s syndrome, CREST and viral infection (centriole/centrosome proteins) |
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Mitotic Spindle Apparatus type 1 (MSA1) |
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| Nucleus | Mitotic | Description | |
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Resting cells:
Mitotic region: Disease (antigens): |
With pure MSA1 antibody there is no specific staining but can occur with other ANAs and only visible in mitotic Staining of the mitotic spindle fibres originating from centroles forming “two opposing open umbrellas” Clinically non specific, inflammatory phase (NuMA) |
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Mitotic Spindle Apparatus type 2 (MSA2) or Midbody |
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| Nucleus | Mitotic | Description | |
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Resting cells:
Mitotic region: Disease (antigens): |
With pure MSA2 antibody there is no specific staining of the nucleoplasm but can occur with other ANAs and only visible in mitotic The junction of two cells usually in telophase becomes highly visibleRA, inflammatory conditions (fibrillar synthesis protein of nuclear membrane) |
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Proliferating Cell Nuclear Antigen type 1 (PCNA) |
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| Nucleus | Mitotic | Description | |
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Resting cells:
Mitotic region: Disease (antigens): |
In the resting cell there is an absence of staining except for nuclei of S-phase in which staining ranging from fine to coarse speckle. This region is negativeSLE, lymphoma, lymphoproliferative disease (Cyclin I) |
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Proliferating Cell Nuclear Antigen type 2 (PCNA) |
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| Nucleus | Mitotic | Description | |
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Resting cells:
Mitotic region: Disease (antigens): |
Similar staining to PCNA type 1 (above) except for the mitotics.
Dots in the mitotic are characteristic of this antibody Various carcinomas |
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