Nucleolar Clumpy
Nucleus	Mitotic	Description
	Negative	Resting cells: Nucleoli: Mitotic region:Disease (antigens):	Nucleoplasm may contain some fluorescence Tight clusters of granules in the nucleoli. NegativeScleroderma (fibrillarin , U3-nRNP)
Nucleolar Homogenous
Nucleus	Mitotic	Description
	Negative	Resting cells: Nucleoli: Mitotic region:Disease (antigens):	Nucleoplasm may contain some fluorescence Uniform staining of the nucleoli NegativePolymyositis / Scleroderma overlap syndrome (PM / Scl)
Nucleolar Speckled
Nucleus	Mitotic	Description
		Resting cells: Nucleoli: Mitotic region:Disease (antigens):	Nucleoplasm may contain some fluorescence fine discrete speckling are seen in the nucleoli NegativeScleroderma (Scl 70 (topoisomerase 1)
Nucleolar multi-dots
Nucleus	Mitotic	Description
		Resting cells: Nucleoli: Mitotic region:Disease (antigens):	Nucleoplasm stained masking a nucleolar  pattern Nucleoli are stained but masked. Few irregular dots seen in the mitotic cellsScleroderma (Nucleolar Organising Region 90 (NOR 90))