HEp2: Cytoplasmic Patterns
Actin (smooth muscle)
Cytoplasm	Mitotic	Description
		Resting cells: Nucleoli: Mitotic region:Disease (antigens):	Microfilaments (actin stress fibres) in the cytoplasm are stained Negative NegativeOften found in autoimmune liver disease 34 kDa actin subunit of microfilament
Cytokeratin (smooth muscle)
Cytoplasm	Mitotic	Description
		Resting cells: Nucleoli: Mitotic region:Disease (antigens):	Intermediate filaments – bundle of stringy coarse fibres Negative NegativeNon-specific, autoimmune rheumatic disease and inflammatory disorder Cytokeratin 8, 18 and 19
Golgi
Cytoplasm	Mitotic	Description
		Resting cells: Nucleoli: Mitotic region:Disease: (antigens):	Perinuclear cytoplasmic speckling confined to one pole. ANA negative. Negative Speckling disperses during mitotic division.SLE and Sjögren’s syndrome (Proteins components of the golgi apperatus)
Jo1
Cytoplasm	Mitotic	Description
		Resting cells: Nucleoli: Mitotic region:Disease: (antigens):	Perinuclear halo with fading of the intensity away from the nucleus. Negative Speckling disperses during mitotic division.Polymyositis (Jo1)
Mitochondrial
Cytoplasm	Mitotic	Description
		Resting cells: Nucleoli: Mitotic region:Disease: (antigens):	Network of cytoplasmic fibres with speckles. Negative NegativePrimary Biliary Cirrhosis (M2 antigen most common, pyruvate dehydrogenase complex)
Tropomyosin
Cytoplasm	Mitotic	Description
		Resting cells: Nucleoli: Mitotic region:Disease: (antigens):	Cytoplasmic microtubules staining seen Negative NegativeNon-specific, rheumatic disease and inflammatory disorder (Tropomyosin)
Vimentin
Cytoplasm	Mitotic	Description
		Resting cells: Nucleoli: Mitotic region:Disease: (antigens):	Staining of intermediate filaments in the cytoplasm Negative Dots in the cytoplasm of the mitotic cellsNon-specific, chronic rheumatic and inflammatory disorders (Vimentin, alpha helix)